A Novel Approach to Capture Large, Variable Cells Using the Invitrogen Bigfoot Spectral Cell Sorter

New methods to enable diverse and challenging flow cytometry applications

Sorting very large cells with standard nozzle tip sizes on cell sorting flow cytometers can be challenging. This is primarily due to the scarce frequency of large cells and their fragile nature. The required pressures for 70, 100, and even 120 μm nozzle tips may compromise large or delicate cells due to the shearing forces associated with smaller orifices. Megakaryoblasts can be rare within a sample so accurate sorting and preservation of viable cells are of the upmost importance.

This resource describes the use of the Invitrogen Bigfoot Spectral Cell Sorter for identification and sorting Megakaryoblasts in complex samples. In addition to the previously available nozzle tips, a larger 200 μm tip was engineered and tested for improved cell viability performance. The methods described are useful for sorting large, complex cell preparations with high viability for downstream applications.

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