Absorbance and Fluorescence Quantification: An Application Guide to Sample QC

Practical insights into leveraging absorbance and fluorescence techniques for accurate sample quantification and quality control (QC)

• Ideal for rapid and accurate measurement of nucleic acids and proteins.
• Provides valuable data on sample purity, including detection of
  contaminants.
• Suitable for highly concentrated, purified samples.

• Best for detecting low-abundance samples below UV-Vis absorbance thresholds.
• Employs secondary fluorophore reporters for enhanced sensitivity.
• Effective in the presence of contaminants or buffer elements that interfere with absorbance measurements.

• Recommendations on when to use absorbance, fluorescence, or a combination of both.
• Strategies for optimizing QC workflows based on sample type and experimental needs.