Unlock Precision: Improve Single-Cell Counting with Countess™ 3 FL

The ideal solution for accurately counting cells and nuclei, evaluating their viability, and assessing aggregation.

Single-cell RNA sequencing (scRNA-Seq) and single-cell ATAC sequencing (scATAC-Seq) rely on precise single-cell counts to generate high-quality data. However, accurate enumeration is challenging due to the presence of cellular aggregates and dead cells, which can compromise experimental outcomes.

The Invitrogen™ Countess™ 3 FL Automated Cell Counter addresses these challenges by providing reliable cell and nuclei counting, viability assessment, and aggregation analysis.

This application note outlines best practices for using the Countess 3 FL to optimize single-cell sequencing workflows, ensuring accurate and reproducible results.

Key touch points include:

Protocols for assessing cell aggregates in brightfield mode
Protocols to ensure accuracy in fluorescence-based counting mode
Methods to ensure ideal gating, speed, and concentrations for cell counting
Protocols for the preparation of single-cell nuclei
And more

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