Data-Driven Bioprocess Optimization Scale-Up

Leveraging real-time monitoring of key media components to streamline bioprocesses and reduce costs

CHO is the most widely used and well-understood cell type for monoclonal antibody (mAb) production. During CHO bioprocessing, each cell line and clone has its own nutritional requirements. In many fed-batch approaches, the cell culture media, basal, and feed, are designed to supply enough nutrients for the majority of cell lines. Overfeeding, however, may lead to the accumulation of toxic metabolites, such as lactate and ammonium, which can contribute to the high cost of production and cost of goods sold (COGS)

An ideal bioprocess solution would provide real-time measurement of nutrient consumption and metabolite generation to maximize cell viability and yields, while reducing costs.

This application note investigates use of the REBEL at-line analyzer for CHO process optimization. Areas of exploration include:

• The impact of fast, at-line spent media analysis on bioprocessing time
• The effects of real-time metabolite analysis on titer, cell viability, and process performance
• The result of nutrient and metabolite management on the costs of goods.
• Considerations for scale-up

Download the application note to gain valuable insights into data-driven bioprocess optimization designed to help you reach process development goals while minimizing complexity and costs.

DOWNLOAD THIS FREE APPLICATION NOTE TO LEARN MORE

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