Data-Driven Bioprocess Optimization Scale-Up

Leveraging real-time monitoring of key media components to streamline bioprocesses and reduce costs

In many CHO fed-batch approaches, the cell culture media, basal, and feed, are designed to supply enough nutrients for the majority of cell lines, but overfeeding may lead to the accumulation of toxic metabolites, plus contribute to the high cost of production. This application note explores utilizing a lower-than-recommended concentration of a commercially available feed to reduce cost of goods and make the process more robust by improving the toxic metabolite profile while maintaining or increasing titer.

The process optimization and scale-up experiments in this application note were powered by the REBEL Amino Acid Analyzer for rapid, at-line spent-media analysis. In addition, the MAVEN on-line glucose and lactate analyzer was utilized at a later stage of the process. The combination of these analytical tools provided unique insights and enabled a data-driven optimization of a bioprocess to reach process development goals with regards to titer, toxic metabolite profiles, and viability.

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