Exploring Key Metabolic Differences in Manufactured CAR T-Cells

Novel reagents for consistent manufacturing of CAR T-cells with well-defined phenotypes are needed to improve treatment outcomes in patients. Traditional ex vivo expansion reagents provide only TCR activation and co-stimulation. Recently developed multi-cytokine backpacks (MCBs) have been shown to provide CAR T-cell manufacturing with additional cytokine support, critical for cell expansion, differentiation, and the selection of potent, long-lived phenotypes.

In this application note, we explored different combinations of immunomodulating proteins during creation of a MCB library. The REBEL from 908 Devices was then used to perform AA analysis to assess differences in the metabolic activity in these activated cells.

Download the application note to learn more about REBEL and the ability to identify distinct correlations between metabolic activity and CAR T-Cell activation and efficacy.